D2O adaptation, Microorganisms, Water Type Effects on Organism Growth, Yeast

E. coli cell morphology: From D2O Adaptation 1

December 5, 2012 Anthony Leave a comment

D2O raised e. coli, grown in 99% D2O YPD. 60x magnification.

D2O e. coli grown in D2O YPD, 10x magnification.

E. coli raised in D2O, and grown here in DDW YPD. 60x magnification.

E. coli raised in D2O, and grown here in DDW YPD. 10x magnification.

More wt yeast in D2O YPD. 60x magnification.

WT yeast grown in 99% D2O YPD.

Wt yeast in D2O at 10x magnification.

Back in the day I was calling the bacteria grown here D2O adapted yeast. Boy was I stupid. Anyway, here is the data I took when trying to compare the morphologies. Despite my naivety, I think there can be interesting studies done with E. coli in D2O based on these experiments and the colony morphology experiments I did.

Also I’ve included actual yeast in D2O images, which show that yeast form “chain gangs.” Basically the buds never seal off and grow new buds and large clusters of bud chains grow. Hopefully I can analyze this more in the upcoming experiments.

Do you?

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