D2O Adaptation day 3

Sample readings

Tomorrow I’m going to inoculate a larger portion of the original sample into a new 99% D2O YPD sample. And I’ll do the same with the 50/50 sample. I want to go to a larger inoculation volume (right now I”m doing 10ml) of about 25ml, but I need to order more water. That will have to wait until Monday.

Also, I have a suspicion that the initially cold YPD may be hampering the Day 1 growth of all samples, so I’m leaving my YPD sealed, but out of the fridge. And we’ll see what happens with the new samples, that I start tomorrow, come Monday.