The 48h sample reading is 0.997- recall the 24h sample reading of yeast in DI water was 3.05, three times higher absorption!

I replaced that sample in the incubator to measure again tomorrow. I also inoculated a new D2O sample from that one and will record the 24h and 48h growth of cells in that batch. Finally I inoculated some cells (from the 48h D2O sample) and placed them in a 50/50 mix of D2O and DDW. I’ll record those values tomorrow as well.