DOI: 10.15200/winn.142722.23341 provided by The Winnower, a DIY scholarly publishing platform
RC4: Day 1
Tag Archives: arabidopsis
DDW3: Day 9 (DI and Tap)
As explained earlier, this is the post of di and tap water samples. The first day of ddw and simulated water samples will come tomorrow. Right now some seeds in these samples are floating and some are at the sample bottom making it hard to get a picture of all the samples. Once the seeds absorb enough water, they will sink and I’ll be able to align them for imaging.
Repeating Crumley 4: The Setup
I’m going to keep this post short and sweet since the setup is nearly identical to the setup for RC3. That can be found here (nice and big link for visibility). Here are the big changes to the setup:
- I used Viriginia Gold #1 tobacco seeds because I forgot I used the totally awesome Cuban cigar seeds in the last batch. Bad Anthony…
- I also used Columbia arabidopsis seeds.
- I did not count 30 seeds per sample. This time I poured seeds and counted until the number of seeds per sample was over 30. This way I can try and enhance the number of seeds in the middle of the sample. This was very helpful with the arabidopsis setup, because the seeds are much smaller than the tobacco seeds and have more attraction/repulsion from the analyslide and weigh paper. As I found out the hard way, something like 60% of the seeds would end up in the sample container and the rest would disappear into oblivion.
- I omitted the DDW samples and will not take any pictures of the control, but will note if anything extraordinary happens in this sample. I figure it is really boring to see pictures of water everyday. I omitted the DDW samples so I can make room for the arabidopsis samples (because I can only fit 8 samples right now).
- There are 4 samples per organism. DI, 33% D2O, 66% D2O, and 99.9% D2O, just as Crumley intended.
Originally, I had Alex (this person again? Who is he/she?) help me with the seed counting on Monday. I realized that I was out of D2O and ordered some. I didn’t get the D2O until yesterday and the presorted seeds had been left out over 24 hours. Since seeds may start sprouting or doing things in light and air, I trashed those seeds and started anew.
I assure you that I used D2O this time. Let’s repeat Crumley! (Day 1 pics to come later today.)
DOI: 10.15200/winn.142722.23338 provided by The Winnower, a DIY scholarly publishing platform
DDW3: Day 8 (yesterday) (DI and Tap)
I included the pictures of the no growth seeds. By now these seeds should have sprouted, which is why I think that I may have used D2O instead of DDW. Hopefully I corrected that today with the new samples. I’m still filing them under DDW3 and don’t care what you think.
Ok I do care. Please don’t cry. I love you…
DDW3: DDW Redo
As I mentioned yesterday, there is a potential D2O contamination so today Alex and I (wait, who’s Alex?) redid the DDW and the simulated water samples with DDW. We’ll check in on the growth process from time to time starting tomorrow.
I think I’m going to separate the posts into days by water type. By this I mean I will post DDW and simulated water as separate posts than the tap and DI water and label their development accordingly. Perhaps something like “DDW3: Day X (Tap and DI)” as an example.
Which reminds me, the next update will come this morning. I took pictures last night, but had to run out of the lab rather hastily. So I will post those pictures today.
DDW3: Potential Contamination
So the seeds in the DDW have yet to sprout. I’m thinking that I accidentally used D2O instead of DDW. I’ll set up the DDW experiments tomorrow and chart their growth. I’ll maintain what I have now in case the seeds sprout, but I doubt it. I’m also going to post on all the DDW3 so far to alert readers and future me that the DDW samples may in fact be D2O samples.
DDW3: Day 4
I have to admit how surprised I am that the arabidopsis seeds have grown so quickly. I guess this is why they are so useful for genetics experiments.
No pictures this weekend.
DDW3: Day 3
DDW3: Day 2
I almost didn’t post these pictures, because nothing had happened yet. Then I looked at the tap water sample of arabidopsis seeds.
What’s that there?
Oh a sprout! Let the experiment begin!
DDW3: Day 1
Today is much better from a setup standpoint. The seeds have soaked adequately and have sunk to the bottom. I tilted the cuvettes for a little so that all the seeds would be as close to the same focal plane as possible (at the front of the cuvette). Pictures taken indicate this.