Category Archives: Water Type Effects on Organism Growth

Dissertation Plan

Here is my dissertation and defense plan. My research is composed of three areas:

  1. Shotgun DNA Mapping
  2. D2O Effects
  3. Open notebook science

All of it will be tied together because of ONS and D2O. Let’s get to it!!!


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Yeast morphology in 80% D2O

Yeast Colonies in 60% D2O YPD

So I suck at my own microscopy technique of taking pictures and combining them. In each case I left out a pretty sizable portion of the colony. 🙁 Oh well. I’m going to do these experiments again (cause I made 2 sets of each type of solid media, 0%-100% D2O) and will be taking new images then. Can’t wait!!

Arabidopsis growth update

Ever since I added a timed incandescent bulb to the system, the plants have been flourishing! That’s a gross over exaggeration, but they’ve been growing faster and better than prior. Check them out! I think next week I’ll add some 20% D2O plants and start charting their growth.20130109-125256.jpg

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80% D2O YPD Solid Media

  1. Water (already prepared and antibiotic added):
    • 32ml D2O YPD
    • 8ml DI YPD
  2. 0.8g agar
  3. Stir and heat on hotplate
  4. Pour 15-20ml into petri dish and allow to cool
  5. seal with parafilm