It says:
- S. cerevisiae
- g160/2d
- 6/12/2010
Yeast specimen
Category Archives: DDW Effects On Life
DDW5: Water Day 15
Yeast: Day 2
So my overnight culture grew, that’s cool. The E coli did not though. That’s not. To make matters worse, the -80C freezer had some weird malfunction and it was nearly RT in there. Hopefully nothing is ruined. I’ll restart experiments on Monday and make new batches of yeast and e coli spreads and liquid media as well. Then I’ll make some glycerol stocks so that I can be ready for all eternity.
On top of all that I’ll reacquaint myself with doing transformations and purifying plasmids because there are some vectors that I need to replenish. Not for the purposes of these experiments, but just because.
I’ll try and do some preplanning over the weekend and through Monday.
Yeast cultures
We streaked two plates and poured a bunch of stock into the third plate (hence the whole lotta cells). I didn’t do the streaking because they weren’t my cells.
I did pick a colony from the third plate and streaked that onto a new plate, so hopefully there will be good spreading.
I parafilmed the plates and stuck them in the fridge.
Onward to tomorrow.
The bread is ready
The yeast has spread into colonies (yay), but the E. coli have not. I don’t get what is happening there, but I’ll try growing them in liquid media and seeing what happens. Tomorrow I’ll check on the e. coli and decide what to do from there. In the mean time, I’ll also start a liquid media batch of yeast to grow side-by-side with the e. coli.
Let’s get it…
Baking Bread!
and poop to some extent…
Ahem! I started cultures of e. coli and s. cerevisiae streaking cells on some plates to be incubated overnight and beyond. From here I will pick colonies and grow flasks of them to get familiar with the growth rates so I can take data. If all goes according to plan, I’ll be getting some preliminary results by the end of the week!
Here is some info about my cells:
E. coli: XL1-Blue MRF’ competent cells that are designed to replicate pBluescript
Yeast: S. cerevisiae of the variety g160/2d
DDW5: Water Day 10
DDW5: Water Day 5
DDW5: Water Setup Part 2
I removed the seeds from the fridge and decided to keep them in a styrofoam cuvette case. This should keep light and temperature pretty stable in the lab. I then put tape over like groups of seeds so I can easily remove the cuvettes and set them up for picture taking.
Believe it or not, that little trick saves me a load of time.
DDW5: Water Day 0
Wow, that sucked. I had to crop, rename, and label 42 pictures for this notebook. Stupid open science movement. Why do I strive to be a good scientist? Why can’t I just let myself be lazy and allow myself to be a bad scientist? Who cares about accurate data and organization?
I do unfortunately.
Anyways here are the Day 0 pictures. Luckily I don’t have to do this every day, that would suck. From now on I may label the water types as follows:
- RODI – Easypure RoDI water from the lab
- CHTM – DI water from the CHTM plant
- SMB – Sigma Molecular Biology DI water
- STC – Sigma Tissue Culture DI water
- DDW – deuterium depleted water
- DDDW – 1% D2O in DDW
Since the last time I did this, I have learned a ton about proper exposure with a camera. And I actually know what aperture, ISO, and shutter speed are for. I’ll do a post that explains all that but in the mean time here are the settings I used for these pictures and those in the future:
- Aperture – Set at F11
- Shutter Speed – Set at 1/13″ (I think, it’s a Nikon camera so it just says 13)
- ISO – Set at 1600
I’m sure these settings are saved with the images so if you download the pics you’ll see the camera settings in photo software.