Results:

• d2o yeast gen 37 – 3.079 at 24f
• d2o yeast gen 38 – 0.422 at 0h
• ddw yeast – 3.039 at 24h
• ddw yeast – 0.312 at 0h

Sorry for the sloppiness, but I’m doing this via my phone.

Also yesterday I grew cells from the inoculating loop, and in 24h they grew to an absorbable of: 2.708 (d2o yeast) and 1.700 (ddw yeast).

I inoculated new samples by mixing 9ml of ypd with 1ml of the previous generation’s culture (from the inoculating loop cultures). I also inoculated yeast on solid media. There are 4 samples:

• d2o yeast on d20 agarose ypd
• d2o yeast on regular agar ypd
• ddw yeast on d2o agarose ypd
• ddw yeast on regular agar ypd

The intent is to compare colony growth on plates over time to see if there are differences between the environments.