Unbeknownst to most of the world, I’ve had a secret agent specially trained to carry out my experiments while I’ve been away. Before I left for Seattle, I trained my lab colleague Nadia to do the 24 hour yeast adaptation experiments. She’s been keeping the experiment up to date and posting the results in the Google Doc I set up back on Day 1. THANK YOU NADIA!!! You can see the updates at that link or just read down:
This morning is my first day in the lab since my departure and I’m picking up right where I left off, the 27th Generation of yeast in the table above are my first recorded values based on Nadia’s efforts.
Here are the results in typical fashion:
- 50% yeast (Gen 26) – 2.934 at 24h
- 99% (gen 26) – 2.862 at 24h
- 50% (gen 27) – 0.536 at 24h
- 99% (gen 27) – 0.442 at 24h
The experiment was setup in typical fashion:
- 9ml of 50% mix of DDW YPD and D2O YPD; 9ml of 99% D2O YPD
- 1ml of 50% DDW YPD yeast added to 9ml of 50% D2O YPD; 1ml of 99% D2O yeast added to the 9ml of 99% D2O YPD
- put in incubator at 30C