Tag Archives: failed

RC2, Water Type Effects on Organism Growth

RC2: Day 8

September 28, 2011 Anthony Leave a comment

Since the lab is a blistering 95F, I’m officially ending this experiment. I will still take pictures for about 7 more days because I want to watch the evaporation rate, but none of this data will be counted towards anything. Most tellingly is that the seeds in DI water barely sprouted and there hasn’t been much change in the past couple of days. Compare that to the DDW seeds and that spells problem…

DOI: 10.15200/winn.142721.17166 provided by The Winnower, a DIY scholarly publishing platform

RC2, Water Type Effects on Organism Growth

RC2: Day 7

September 27, 2011 Anthony Leave a comment

DOI: 10.15200/winn.142721.17161 provided by The Winnower, a DIY scholarly publishing platform

DDW2, Open Notebook, RC2, Water Type Effects on Organism Growth

CHTM Cooling System Down

Aside September 26, 2011 Anthony 2 Comments

Since this past Friday (Sept 23, 2011) the cooling system at CHTM has been down. I’m not sure if it is turned off for the impending winter or temporarily broken. This means that right now the lab is a toasty 92F and has been that way all weekend long. Unfortunately this can potentially mean a lot with regards to my seed growth experiments. Now I’m not sure if this is actually the case, but I think with regards to the experiments it may be in the best interest to scrap Try 2 for both the Repeating Crumley and the DDW Effects on Seed Growth until the temperature becomes a bit more stabilized. Now I will still record data and keep track of the experiments, but I will not include these results in any final markups. But that doesn’t mean I can’t learn something from these experiment!

DOI: 10.15200/winn.142721.17155 provided by The Winnower, a DIY scholarly publishing platform

RC2, Water Type Effects on Organism Growth

RC2: Day 6

September 26, 2011 Anthony Leave a comment

DOI: 10.15200/winn.142721.17151 provided by The Winnower, a DIY scholarly publishing platform

RC2, Water Type Effects on Organism Growth

Repeating Crumley Try 2: Setup

September 26, 2011 Anthony Leave a comment

I am on Trial 2 of the Repeating Crumley Experiment. Trial 1 was a pretty decent success, but I ran into some issues regarding image acquisition, experiment stability, and water evaporation. I made some adjustments and began the experiment again. Here is how I setup the second trial:

There are 20 seeds of the Dark Virginia variety from the Tobacco Seed Company in each sample.
There are 8 water samples: di water, deuterium depleted water, 33% d2o in di water, 66% d2o in di water, 99.9% d2o, 33% d2o in ddw, 66% d2o in ddw, and a sample of di water without any seeds.
The sample of di water with no seeds is acting as a control to monitor the possibility of fungal/mold growth. 20 seeds were added to water and allowed to incubate in solution for 30 minutes. At the end of the incubation period the seeds were removed and the sample was sealed.
The seeds were added to each sample container (analyslides), and a water type was added immediately after seed addition.
After closing the samples, clear nail polish was added around the rim to seal the chambers from the surrounding environment.
Data is taken using a Logitech HD Pro Webcam C910.

In the previous trial, the images were taken from below the samples. This resulted in several sample drops. In this trial, I have placed the samples flat on the lab top and am taking data from above the samples. In this manner, the samples have been significantly less agitated then before.

My biggest worry is that the nail polish has/will contaminate(d) the water samples, producing undesirable results. I have ordered a bunch of supplies to test what may create the best seal, while creating the least amount of interaction with the sample water. That experiment will be started as soon as possible and the result will be used to create a third trial run.

DOI: 10.15200/winn.142721.17145 provided by The Winnower, a DIY scholarly publishing platform

RC2, Water Type Effects on Organism Growth

RC2: Day 5

September 25, 2011 Anthony Leave a comment

DOI: 10.15200/winn.142721.17141 provided by The Winnower, a DIY scholarly publishing platform

RC2, Water Type Effects on Organism Growth

RC2: Day 4

September 24, 2011 Anthony Leave a comment

I haven’t had much time to study these pictures, but from quick observations it looks like the seeds in 33% d2o mixed with ddw are sprouting before the seeds in 33% d2o mixed with di water. Another interesting thing to note is the seeds in pure di water are sprouting slowly. I’m hoping that my use of nail polish to seal the analyslides hasn’t ruined the experiment.

DOI: 10.15200/winn.142721.17136 provided by The Winnower, a DIY scholarly publishing platform

RC2, Water Type Effects on Organism Growth

RC2: Day 3

September 23, 2011 Anthony Leave a comment

It should be noted that I shifted all the seeds around in most of the samples so there was a decent distribution in the center of the sample as opposed to being near the edges and near the meniscus. Hopefully everything will remain like this and I won’t have to ever touch the samples for the next 10 days.

DOI: 10.15200/winn.142721.17131 provided by The Winnower, a DIY scholarly publishing platform