The digestion is complete. There is really no way for me to verify the results so I just went ahead and did an enzyme cleanup and purified the DNA. Now I have:
- Tube 1 – 170.0ng/ul –> 63.75 nM
- Tube 2 – 178.3ng/ul –> 66.86nM
Once I verify the pBR322 digestion and gel extract (coming up next) I can set up the ligation reaction and finish up!