D2O Adaptation Day 47

So I let the yeast from Friday incubate over the weekend, then there was a power failure. Hopefully nothing major happened. Because of the power failure I’m postponing my morphology experiments for either tomorrow or Wednesday and also the D2O solid culture growth until tomorrow. In the mean time we have some data:

  • D2O yeast (generation 36) – 3.261 at 72h
  • D2O yeast (gen 37) – 1.019 at 0h

As per usual, 9ml of D2O YPD were mixed with 1ml of D2O yeast from generation 36. I also did 10ml of D2O YPD with a colony of yeast inoculated from the same sample (gen 36). Hopefully that batch minimizes the amount of cells affected by the power outage.