Via figshare:

Yeast Growth at RT in D2O and DI water. Anthony Salvagno. Figshare.
Retrieved 18:10, May 23, 2012 (GMT)
hdl.handle.net/10779/736ebb6f977250f55e9d2bbb12f4f796

First let me explain the experimental setup:

• This is a carry over from yesterday’s experiment. With the setup here.
• Every hour I took data from the cultures I inoculated. I put 500ul in semi-micro cuvettes to measure in the nanodrop.
• After I take data, typically I store the measured cuvettes at RT on the lab top in a cuvette holder (the cuvettes are sealed with PE caps).
• Originally I did this because I wanted to take a picture to compare the growth after the full experiment was completed, but I noticed with e. coli that the cultures continue to grow at RT, so a picture wouldn’t reveal anything or be accurate.
• After this experiment I decided to use the nanodrop to compare the growth at RT from all the samples (with the more recent samples having less time to incubate at RT for obvious reasons).

Linked above is the results of the experiment with the excel file I used to compile the data.

Now it’s time for some notes:

• Originally I had compared the growth of these samples to the original data. But realized that the comparisons weren’t valid because each sample grew over 5 hours and the samples were independent of each other at this point.
• It is interesting to note that the D2O sample is almost the same in every sample, except in hours 4 and 5 where the growth is more from incubation at 30C than RT (~25C). But even those two data points are almost identical.
• The DI sample has more steady growth in each sample. I should compare these values to what they were when they were removed from the incubator.

I don’t know if this data is useful but I thought it was interesting so I thought I’d share it with the world. If you have any suggestions for this particular experiment let me know (comment, twitter, email, snail mail, etc)!