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Repeating Crumley: 33% D2O Growth vs Time
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Tag Archives: repeating crumley
Repeating Crumley: 66% D2O Growth vs Time
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Repeating Crumley: 99.9% D2O Growth vs Time
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Repeating Crumley: Seed Growth vs Time
As you know I posted pictures of the seed growth every day. Well a couple of days ago I decided to organize the pictures by sample in one post as opposed to by day. This way you can see the growth of each sample over the 15 day period. The posts are already posted but I figured I would compile the links here with some commentary so you know what’s up.
As you can see trial one wasn’t a huge success in this regard because every so often I would shift the seeds around. It makes it hard to track each individual seed, but with that said there are some obvious things you can take away:
- You can really see the growth over time. It’s hard to keep track on any given seed in each sample, but on an average you can really get a feel for how quickly these seeds develop compared to the other samples (ie the stuff in di water grew first compared to the stuff in 66% D2O).
- You can track the evaporation rate decently enough. This was a major problem towards the end of the experiment and you can really see its effect.
Overall I’d say I did a decent job with this experiment given that it was a first try. I’ve managed to improve some things for Try 2 and I’ll be making more improvements when it comes to Try 3, which hopefully will be good enough for an official experiment.
BTW: The plugin I used to create the slideshows is called Portfolio Slideshow.
DOI: 10.15200/winn.142534.42459 provided by The Winnower, a DIY scholarly publishing platform
RC2: Day 4
I haven’t had much time to study these pictures, but from quick observations it looks like the seeds in 33% d2o mixed with ddw are sprouting before the seeds in 33% d2o mixed with di water. Another interesting thing to note is the seeds in pure di water are sprouting slowly. I’m hoping that my use of nail polish to seal the analyslides hasn’t ruined the experiment.
DOI: 10.15200/winn.142721.17136 provided by The Winnower, a DIY scholarly publishing platform
RC2: Day 5
DOI: 10.15200/winn.142721.17141 provided by The Winnower, a DIY scholarly publishing platform
Repeating Crumley Try 2: Setup
I am on Trial 2 of the Repeating Crumley Experiment. Trial 1 was a pretty decent success, but I ran into some issues regarding image acquisition, experiment stability, and water evaporation. I made some adjustments and began the experiment again. Here is how I setup the second trial:
- There are 20 seeds of the Dark Virginia variety from the Tobacco Seed Company in each sample.
- There are 8 water samples: di water, deuterium depleted water, 33% d2o in di water, 66% d2o in di water, 99.9% d2o, 33% d2o in ddw, 66% d2o in ddw, and a sample of di water without any seeds.
- The sample of di water with no seeds is acting as a control to monitor the possibility of fungal/mold growth. 20 seeds were added to water and allowed to incubate in solution for 30 minutes. At the end of the incubation period the seeds were removed and the sample was sealed.
- The seeds were added to each sample container (analyslides), and a water type was added immediately after seed addition.
- After closing the samples, clear nail polish was added around the rim to seal the chambers from the surrounding environment.
- Data is taken using a Logitech HD Pro Webcam C910.
In the previous trial, the images were taken from below the samples. This resulted in several sample drops. In this trial, I have placed the samples flat on the lab top and am taking data from above the samples. In this manner, the samples have been significantly less agitated then before.
My biggest worry is that the nail polish has/will contaminate(d) the water samples, producing undesirable results. I have ordered a bunch of supplies to test what may create the best seal, while creating the least amount of interaction with the sample water. That experiment will be started as soon as possible and the result will be used to create a third trial run.
DOI: 10.15200/winn.142721.17145 provided by The Winnower, a DIY scholarly publishing platform
RC2: Day 6
DOI: 10.15200/winn.142721.17151 provided by The Winnower, a DIY scholarly publishing platform
CHTM Cooling System Down
Since this past Friday (Sept 23, 2011) the cooling system at CHTM has been down. I’m not sure if it is turned off for the impending winter or temporarily broken. This means that right now the lab is a toasty 92F and has been that way all weekend long. Unfortunately this can potentially mean a lot with regards to my seed growth experiments. Now I’m not sure if this is actually the case, but I think with regards to the experiments it may be in the best interest to scrap Try 2 for both the Repeating Crumley and the DDW Effects on Seed Growth until the temperature becomes a bit more stabilized. Now I will still record data and keep track of the experiments, but I will not include these results in any final markups. But that doesn’t mean I can’t learn something from these experiment!
DOI: 10.15200/winn.142721.17155 provided by The Winnower, a DIY scholarly publishing platform
RC2: Day 7
DOI: 10.15200/winn.142721.17161 provided by The Winnower, a DIY scholarly publishing platform