Arabidopsis seeds in DI water (0.0156% D2O).
Arabidopsis seeds in 33% D2O.
Arabidopsis seeds in 66% D2O.
Arabidopsis seeds in 99% D2O.
Growth of tobacco seeds in DDW (0.0001% D2O).
Growth of tobacco seeds in DI water (0.0156% D2O).
Growth of tobacco seeds in 1% D2O.
Tobacco seed root length growth.
Growth of tobacco seeds in DI water.
Growth of tobacco seeds in DDW.
Growth of tobacco seeds in 33% D2O.
Growth of tobacco seeds in 66% D2O.
Growth of tobacco seeds in 99% D2O.
The plants are one week old! They seem to be doing really well, for the most part. As expected, as the concentration of D2O increases the plants develop slower. Also as expected the plants also exhibit leaf decolorization. Notice how in 60, and 70% D2O the leaves are a pale green. In 80% the plants are too small to notice growth. It also seems that the plant in 5% D2O is growing the fastest, which seems to be in line with my observations from the last trial run, and also with hypotheses from my dissertation (to be posted as soon as it is all finished). I will have to record some observations of the plants in DDW and compare them to 5% D2O and 10% D2O.
I just finished some beautiful graphs by combining the aggregation data sets from the protein aggregation studies (Ovalbumin and Catalase). Check them out:
ovalbumin visual aggregation: (left) non-aggregated sample, (right) after aggregation
plot of ovalbumin aggregation
Plot of catalase aggregation