Tag Archives: data
Arabidopsis Growth Try 4: Week 3
Growth of Arabidopsis in heavy water
DOI: 10.15200/winn.142722.25163 provided by The Winnower, a DIY scholarly publishing platform
Growth of tobacco seeds in low concentrations of deuterium
DOI: 10.15200/winn.142801.10415 provided by The Winnower, a DIY scholarly publishing platform
Growth of Tobacco Seeds in Heavy Water
DOI: 10.15200/winn.142722.25159 provided by The Winnower, a DIY scholarly publishing platform
Arabidopsis Growth Try 4: Week 2
The plants are one week old! They seem to be doing really well, for the most part. As expected, as the concentration of D2O increases the plants develop slower. Also as expected the plants also exhibit leaf decolorization. Notice how in 60, and 70% D2O the leaves are a pale green. In 80% the plants are too small to notice growth. It also seems that the plant in 5% D2O is growing the fastest, which seems to be in line with my observations from the last trial run, and also with hypotheses from my dissertation (to be posted as soon as it is all finished). I will have to record some observations of the plants in DDW and compare them to 5% D2O and 10% D2O.
Protein Aggregation Graphs
I just finished some beautiful graphs by combining the aggregation data sets from the protein aggregation studies (Ovalbumin and Catalase). Check them out:
Feb 28 Yeast Growth
I’ve been scratching my head about this for the past two days. I could have sworn that I did a measurement of yeast growth after adaptation and it turns out I did. Here is the data:
I’ll graph this data and publish that when I’m done (after I defend).
Tobacco Seed Growth Rates
I got some awesome new data to show. The first is the compilation of all the Repeating Crumley experiments. And the second is some new data that I’ve been meaning to create and now have with the help of Koch.
The data above is the compilation of all the RC data. Each trial had different water types, but I combined the samples that were the same in every set (DDW, DI water, 33% D2O, 66% D2O, and 99% D2O). Steve adapted his R-code that applies binomial confidence intervals to a data set and used it on this data. If that makes no sense, then just know that the dotted lines are the most probable range of germination rates. For instance, in 66% D2O there is a ~70% likelihood that seeds will germinate at a rate within the dotted yellow lines.
Now it’s time for some brand new data:
Here we went through the pictures from Trial 5 and compared the growth rates of the roots. We calculated the lengths of various seeds in each image and tracked the changes from image to image. We chose DI, DDW, and 1% D2O, because the D2O concentrations are relatively similar and because we wanted to test a hypothesis from a while ago. It’s interesting that the seeds in DDW and D2O grow at the same rate, while seeds in DI water grow at roughly half the speed.
WT E. coli colony (on D2O LB agar) morphology
Yesterday I posted some pictures of E. coli colony morphologies. This was one of the colonies, but it wasn’t as developed, so today I’m adding the extra day’s growth image.
Looks great! It’s interesting to note that the colonies grown on D2O agar grow out. Instead of getting thick like it normally does, it grows in an outward direction. I guess I would attribute that to the stress induced by being in D2O.
Comparing the results from today to WT E. coli grown onĀ DI media and D2O adapted E. coli grown on D2O media, it seems there is an interesting mix of morphological behavior. The adapted E. coli is very “brainy” and obviously the normal WT is “smooth,” but today’s specimen is in between smooth and brainy. Unfortunately I can’t make out the topographical features because the E. coli (as I mentioned above) is very flat. But the contour is very feature rich.