I asked Koch to take pictures of the plants for me while I am out of town. Here are his notes regarding the experience:
Wasn’t sure of your camera settings. I had trouble with depth of focus, so I chose aperture priority (“A”)
and F=22, which seemed to work OK. I didn’t want to change your zoom, so I didn’t. I took pictures of bottoms
until half way through when I realized to take tops too (so two pictures each for arabidopsis). This was a
mistake, but since I don’t know if the photos are useful, I’m stopping now.
Photos are labeled TYPE CUVETTE_NUMBERS UP/DOWN and I left in the actual photo number, as follows
I can’t tell in the havanna seeds sample, but the virginia gold tobacco seeds in di and tap have tiny hairs. This is not alarming because I noticed this in prior trials. It is obvious that the hair growth in the ddw samples far exceeds that of the growth in these two samples.
Here are the cropped photos. I used a program called JPEGcrops to mass crop the images from day 7. It was sweet and quick. I also included (uncropped) photos of the DI and tap water samples from Trial 3 of the DDW experiment. I don’t know if the curviness is real or not so I’ll let you comment below if you think it’s real. My money is on yes!
I have to say I’m convinced. I’m convinced that the root hairs are a real phenomenon of growing in ddw. All of the tobacco seed samples (havanna and virginia gold) growing in ddw have at least one or two seeds that have root hairs. ALL. The DI and the tap water samples are barely growing hairs. There is a clear difference between these hairs and the DDW samples.
As for the arabidopsis. I don’t know. The only thing I’m noticing is that the stems are growing very crooked. So crooked that most of the seedlings have intertwined. This is tough to confirm because the DI sample barely sprouted, but the tap water sample appears to have longer persistance lengths (to borrow a word from DNA). By this I mean the angle of curvature is greater in the tap water than in all the ddw samples which tend to curve like no ones business.
I can confirm this by comparing to the previous batch of samples. The tap and di water samples had similarly longer curve radii, while the ddw (which had to start after) seeds got all tangled together.
There is at least one seed in every CA sample that has sprouted (in fact in many of them most seeds have sprouted). Not only this but I’d say overall that over the past two days, there is more growth in the DDW samples than there is in either the tap or di water samples. By more growth I mean the length of seedling (radicle).
As far as the tobacco seeds go, there are a few sprouts here and there, but they generally don’t sprout until days 3/4.
As mentioned yesterday I setup the next iteration of the “DDW Effects on Life” experiment. I won’t have any pictures today and maybe not tomorrow because I’m in the process of rebuilding the photography station (need something a little more stable). The first few days aren’t that important anyway, because there are no noticeable phenotypical changes and the root hairs don’t show up for some time.
As for the setup, I’ve made a few changes that I think are for the better. Also, for all product details please see the experimental product page at the top. Let’s get into the nitty gritty:
There are 12 samples per seed type, and there are 3 seed types (2 tobacco, 1 arabidopsis). I’m using a new batch of Virginia Gold (tobacco), Havana 2000 (tobacco), and Columbia arabidopsis. There are 10 samples of DDW, 1 sample of tap water, and 1 sample of DI water (I only have 3 cuvette racks and this is the limit that I can hold for now).
For this experiment I used a new bottle of DDW. The other bottle had been opened since September and it was time for a change (in case D exchange is something to worry about).
Wearing gloves, I counted 36 macro cuvettes and lids. I labeled each lid as either CA, VG, or H for each plant type (12 each). 2 lids were also labeled either tap or DI to distinguish these samples from the DDW samples.
No less than 5 seeds (at most 6 seeds) were poured into each cuvette. I’ve noticed that pouring the seeds from their wax paper pouches is much faster than sorting by hand with tweezers. It also prevents me from crushing the seeds with the tweezers or from contaminating them by touching them to another surface.
3ml of a water type were then added to each cuvette and sealed with the lids. To be clear, I would add seeds to every cuvette (per seed type) and then add water and seal, then move on to the next seed type.
Typically the seeds need to soak for several hours (I don’t have an exact number, but 24 hours works well enough) before they settle on the bottom of the cuvette.