Video 1 – (Download by right clicking and selecting save as ~40MB) This is the dark virginia seeds in ddw as viewed from the front of the cuvette.
Video 2 – (Download by right clicking and selecting save as ~18MB) This is the dark virginia seeds in ddw as viewed from the side of the cuvette.
Video 3 – (Download by right clicking and selecting save as ~25MB) This is the dark virginia seeds in tap water as viewed from the front of the cuvette.
Video 4 – (Download by right clicking and selecting save as ~34MB) This is the dark virginia seeds in ddw as viewed from the front of the cuvette, but I’m wiggling the cuvette holder so you can see the motion of the hairs and differentiate them from scratches on the glass.
Here are all the pictures as promised from yesterdays presults (like that one?). Compare the first picture in the gallery to this one from the last update 15 days ago. Notice the amount of growth in the past 15 days.
While the seeds in ddw have kept growing, it appears the seeds in all other samples have either stopped growing, are growing slower, or are dying. This time most roots did not have hairs or had very little hairs, which is different from last time where the hairs were more obvious. I don’t know what to make of this for now.
I’m also not going to make any conclusions or assumptions in this post or any other observations for that matter. I’ll leave it up to you to think about what’s different and post observations in the comments. The one thing I will say though is that this study is fascinating!
I took some videos of the hairs which I will post in the next post.
Well oh well. Look at what we have here. I’m so excited I can hardly contain myself…
So it’s been a while since I updated and that’s because I’ve pretty much left the seeds to their own accord. I didn’t want to end the experiment officially because I wanted to see if there would be any other startling results, but it was pretty much over. Now I think I got some startling results. Let’s talk about this:
I took pictures of 3 different samples, one dark virginia in ddw, one dark virginia in tap water, and presoaked dark virginia in ddw. The pictures are lit (to see the results) using my new Droid Bionic camera led (for flash) as the lighting source.
Two pictures are of the same sample. One is very well lit and the other is dark, but with some side light (I liked the way it looked artistically so I included it and made it the feature image). In these two images I wanted to illustrate that the seedlings were fully germinated (and probably at max growth submerged in water), but that the root hairs I adoringly refer to as root fro almost have completely filled the solution! What the hell could this mean? I no longer think it is a lack of nutrients. Why? Because this effect is only seen in the samples of ddw. The DI water samples which never had quite as much root fro (but quite a bit) looks to have actually gone into remission, meaning the root fro seems to be getting less.
To contrast that I showed what it is like in a typical sample of tap water. Note that there is root fro in there (the plant on the bottom has its hairs visible) but they are nowhere near filling the sample. Crazy!
To make matters even more crazy, the presoaked seeds in ddw haven’t even germinated properly and there are (is?) root fro everywhere! Both presoaked samples are like this. On top of that it looks like presoaking may have hindered or impaired the germination process because every sample’s growth is stunted. The root fro amounts per sample is typical though (meaning ddw has the most, di water next most, and tap water the least amount of fro).
Holy moley! I don’t even know what to make of this. I’m going to re-setup this experiment in a few days but include D2O (like 33% and 66% amounts) to see if the root hairs are a similar phenomenon and to what extent.
I was going to use this space to compare the two different lenses, but I was so enthralled with the quality of the one 10x lens (from Opteka) that I ended up just taking a bunch of pictures with the one lens and thought I would share it all with you.
I tried to do some reading on the subject over the weekend, but it appears there is a term called root hairs that appear during growth of the primary root (which is what the radicle turns into). Now the books I read were limited in their germination physiology but at least I now have a foothold.
I did note while taking the pictures that in every case, the DDW seeds had the most root ‘fro, then the DI water seeds, and then the tap water seeds had the least. I still think this is a product of lack of nutrients, but there may be something in the question “Why would the root fro be more prominent in deuterium depleted water than in deionized water?” They are both pure, but maybe ddw is more pure than di water? Something to investigate I think.
I encourage you to enlarge every picture up there and see the amazing resolution one attains with the Nikon D40.
Those are images of the first batch of samples (Dark Virginia seeds, not pre-soaked), I’ll take pictures of everything and post it next week. But here are the preliminary reports of what different water types do to tobacco seeds:
Every seed submerged in deuterium depleted water (DDW) sprouted little hairs on the initial root (the radicle). The interesting thing is this happened almost immediately after emerging from the seed coat.
Typically the seeds submerged in deionized water (DI water) germinated the slowest. More will come on this when I replicate the Crumley experiment.
Little hairs sprouted inconsistently on the seeds in tap and DI water but are more prevalent on the DI water seedlings. They are not as abundant on these seedlings as they are on the ddw seedlings. If I had to give an analogy (and I do) then I would say the ddw seedlings can grow a nice ‘fro, while the other seeds exhibit male pattern baldness.
The little hairs remain localized on the end of the root and aren’t distrubted along the hypocotyl (early stem, and I almost said axon, lol!) so I’m inclined to believe that this is an early root system that is developing because of a lack of nutrients in the water (seen on ddw, most DI, and almost no tap water seeds). But I’m no botanist so I’m just guessing. The fact that the hairs are really prominent in the ddw seeds might suggest the plant recognizes the lack of deuterium, but I’m not willing to make that leap yet.
I setup a new photography system for these seeds. Dr. Koch lent me his Nikon D40 dSLR camera and I purchased some magnification lenses for it. I have the camera setup on an optical post and use a cylindrical lens holder to mount the seed samples (in cuvettes). The picture quality is much better now, with a much higher resolution. I’ll be looking into microscope images soon. Soon I’ll be developing a reliable way to measure the germination, but let’s not jump the shark now. All will be revealed in due time.
Also I was going to measure the pH of the samples at this stage of their development to gain some insight into whether the germination event drastically alters the pH, but the probe in the lab is too big and I don’t have enough sample volume. So I’m thinking that next week I combine the volumes of the water (of each type) to do one “average” measurement. There are four samples of each water type, two for each seed species, and each is filled about 2ml which would give me 8mL of combined volume for each water type. I’m just waiting for the pre-soaked samples to reach full germination (ie shed the seed coat). Now I’m not saying this will work, and it may not be reliable, but hopefully it is a decent approximation for expectations for now until I learn a little bit more.
In most of these samples there is one seedling that shed the seed coat, and in few that seedling is floating at the water surface. It also seems that the branching in the second DD water sample (the Virginia Gold species) has been shed on one of the seedlings (perhaps the one that shed the seed coat). There is a stringy thing floating in the background, which I’m not sure is visible in the image but definitely there in the sample.
Note: There is some wordpress gallery error that won’t display the second and third picture captions. They are: 2. Dark V in Tap Water. 3. Dark V in DI Water
These images are from the presoaked tobacco seeds. I soaked them in their respective buffers (deuterium depleted water (DD water), tap water, and deionized water (DI water)), and then stored them in the fridge (4C) for 5 days in case there is any drastic chemical exchange between the seed and the water (which would change the buffer). It appears that the deuterium depleted water has started sprouting first in both cases, which is speculative but noteworthy. Also the branching that I noted in the last post appears again here, but only in the DD water samples, which is interesting. I wonder what that could be.