Yay, good digestion! Here is some useful information about which piece to gel extract. I’m always looking for this mostly for doubling checking purposes, but I need to figure out a place to store this so I can EASILY find it. For now all that matters is I cut out the larger band (top) and do gel cleanup. Onward and upward I suppose…
I extracted into 2 tubes and here are the final amounts:
- 88.7ng/ul –> 52nM
- 101.9ng/ul –> 60nM