New YPD made

I just ran out of my last batch of both DI and D2O YPD. This time I am making the protocol with 500ul of Ampicillin added (460ul added to D2O).

Yesterday I added amp to my 10ml test tube sample and it seems to have killed the bacterial infestation without impacting the yeast growth. Normally when there is bacterial growth, the yeast settles and the bacteria floats in solution. This morning the solution was clear and the yeast was nicely nestled at the bottom.