D2O Adaptation Try 2: Day 1

Because I’m 90% confident that the adapted yeast was actually e. coli (based on images and smell) I’m going to restart the experiment. I started by cleaning the incubator/shaker. Might as well try and minimize e. coli outbreak…

Setup:

  • Add 9ml of D2O YPD (all that I had left) to test tube.
  • Inoculate a colony from yeast grown on D2O solid media.
  • Add 9ml of DDW YPD to test tube
  • Inoculate colony from yeast grown on D2O solid media.
  • Place in incubator at 30C and 185rpm

I also made a fresh batch of D2O YPD:

  • 4.6g of powder YPD
  • 92ml of D2O
  • stir until dissolved
  • filter (2um) into bottle