D2O Adaptation Day 50: D2O Yeast Time Trials – Setup

After some really interesting results from some microscope observation yesterday (to be uploaded shortly), I’m doing some different time trials today. The setup is the same, but today I’m switching the growth media. D2O yeast will be grown in DDW YPD and wt yeast will be grown in D2O. Here’s the setup:

  1. Add 9ml of YPD to 3 test tubes:
    • D2O YPD
    • D2O YPD
    • DDW YPD
  2. Add 1ml of culture to each test tube:
    • 1ml of D2O yeast to D2O YPD
    • 1ml of D2O yeast to DDW YPD
    • 1ml of DDW yeast to D2O YPD
  3. Incubate at 30C and shake
  4. Measure in nanodrop every hour

Yesterday I noticed that the D2O yeast grew more on normal solid media than DDW yeast did (and also more than D2O yeast on D2O solid media). I wanted to get an hourly analysis of this growth. Unfortunately I didn’t have enough made DDW YPD and don’t have time to make a new batch because of timing of the measurements with this afternoon’s activities. So for now I’ll compare this to every other DDW growth experiment. And next week run the trial again with DDW yeast growing in DDW.

I also want to do some microscope analysis of the yeast in the different media because of the observations I made last night. Like I said that data will be up shortly.