D2O Adaptation Day 44 Time Trials Results

Via figshare:

Day 44 Yeast Adaptation Hourly Growth. Anthony Salvagno. figshare.
Retrieved 23:54, Nov 09, 2012 (GMT)
http://dx.doi.org/10.6084/m9.figshare.97284

So based on these results, it appears that there is a difference in growth rates between my adapted D2O yeast and yeast adapted to H2O and grown in D2O. The main evidence is that the absorbance of adapted yeast starts much lower than the nonadapted yeast, and after a couple hours surpasses the cell count of the nonadapted yeast. The nonadapted yeast had exhibited relatively little growth for the first 5 hours of the experiment, while the adapted yeast grew consistently over the duration of the experiment.

The other aspect of this experiment was to compare the growth of yeast in water after different incubation times to determine if the yeast is exhibiting log-phase growth. I would say it most definitely is. Both samples grow at about the same rate, just starting from different cell counts. This also means that the yeast inoculated in D2O was growing at this rate and was severely stressed when placed in the D2O medium.

The next experiments are to compare yeast grown in DDW YPD to yeast adapted and grown in D2O to yeast adapted to H2O and grown in D2O. Hopefully there will be some interesting results. And next from there will be to grow the adapted yeast in H2O to see if H2O causes similar stresses to D2O adapted yeast that D2O causes to H2O adapted yeast.