Results:

• D2O Yeast gen 34 – 3.097 at 24h
• DDW Yeast – 1.241 at 24h
• D2O yeast gen 35 – 0.856 at 0h

Yesterday I did a starter culture and today it’s absorbance is 1.241 in 24 hours. I’m electing to continue incubation of that culture for another 24h and am starting a new culture in 10ml of DDW YPD inoculated via inoculation loop. I also continued the yeast growth of the D2O strain (9ml of D2O YPD and 1ml of culture from generation 34). All three samples are incubating at 30C in my shaker.