D2O Adaptation Day 41

Today’s measurement:

  • D2O yeast (Gen 32) – 3.212 at 24h
  • D2O yeast (gen 33) – 0.675 at 0h

Since I’m still awaiting the arrival of my DDW I can’t get a starter culture of that going yet, so I’m just going to keep adapting the yeast in D2O:

  • 9ml of D2O YPD and 1ml of culture from generation 32.
  • Incubate at 30C

I also had to make a fresh batch of D2O YPD:

  1. measure D2O (92ml in this bottle)
  2. add 4.6g of YPD
  3. stir
  4. using a syringe filter the YPD (don’t autoclave)

Hopefully by tomorrow I’ll have my DDW so I can prepare for a time trial by the end of the week.