Today’s measurement:
- D2O yeast (Gen 32) – 3.212 at 24h
- D2O yeast (gen 33) – 0.675 at 0h
Since I’m still awaiting the arrival of my DDW I can’t get a starter culture of that going yet, so I’m just going to keep adapting the yeast in D2O:
- 9ml of D2O YPD and 1ml of culture from generation 32.
- Incubate at 30C
I also had to make a fresh batch of D2O YPD:
- measure D2O (92ml in this bottle)
- add 4.6g of YPD
- stir
- using a syringe filter the YPD (don’t autoclave)
Hopefully by tomorrow I’ll have my DDW so I can prepare for a time trial by the end of the week.