D2O Adaptation Days 37-40

On Thursday I ordered some more D2O and DDW for use in these experiments (Scifund money!) and I’m awaiting the arrival of the DDW (the D2O arrived on Friday). I ordered 3 bottles of D2O (total $313.36) and 2 bottles of DDW (about $250 total). Since I’m out of DDW and now can’t make any more DDW YPD I’m just going to propogate the D2O yeast since I have plenty of that.

On Friday I inoculated a culture of D2O yeast in 9ml of D2O (from an inoculating loop, instead of my usual 400ul of previous culture). I let it grow over the weekend and the 72h growth is: 3.343. This morning I inoculated 400ul of that culture in 10ml of D2O YPD and  measured that in the nanodrop: 1.072 (0h).