Day 36 D2O Adaptation Time Trial Results

Via figshare:

Day 36 Yeast Adaptation Hourly Growth. Anthony Salvagno. figshare.
Retrieved 16:50, Nov 02, 2012 (GMT)
http://dx.doi.org/10.6084/m9.figshare.97154

The results are in. I finished the experiment with a 24h time point and uploaded to figshare. Yesterday I was planning on doing 6h of time points and then a 24h measurement, but I was running low on sample and wanted to provide as much media as possible so I could rerun the experiment next week with a larger cell count for unadapted D2O, which would provide a better gauge of adaptation.

I’m still not quite sure what to think of this data. I’m going to keep the generations of yeast growing and take more measurements down the road. I’m also going to grab some papers on yeast adaptation and growth rates and see if there is something I can use to better determine if I’ve reached my goal.

I do feel at the very least the cells are more tolerant of D2O than they were a little over a month ago. Up next is some microscope analysis. Hooray!