D2O adaptation, Microorganisms, Water Type Effects on Organism Growth, Yeast

D2O Adaptation Day 35

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Back on track here are the Day 35 results:

  • DDW Yeast – 3.111 at 24h
  • 50% D2O yeast (gen 28) – 2.969 at 24h
  • 99% D2O yeast (gen 28) – 3.007 at 24h
  • DDW Yeast – 1.192 at 0h
  • 50% D2O yeast (gen 29) – 0.606 at 0h
  • 99% D2O yeast (gen 29) – 0.806 at 0h

I set up the next day’s experiment with some added twists. I have a total of 9 samples for tomorrow, 3 samples of each water type:

  1. yeast in 99% DDW YPD
  2. yeast in 99% D2O YPD
  3. yeast in 50% D2O YPD

The first set of samples (1 of each type) is prepared as I normally prepare an experiment: 9ml of ypd with 1ml of ypd with culture.

The second set of samples (1 of each type) is 9ml of each water type with a culture inoculated from the Day 34 samples. By this I mean instead of adding 1ml I added whatever the inoculating sticks can hold and swooshed it around in the sample. The goal of this is to see how much yeast can grow in 24 hours from a small number of cells. In my original starting culture samples, the cells in D2O would grow astronomically slower than in DDW and my assumption is that if my cells are truly adapted the difference in growth won’t be so drastic.

The third set of sample (1 of each type) is 9ml of each water with a culture inoculated from glycerol stocks I made of samples from the first week of experiments. All the samples are inoculated from DDW grown yeast. The purpose of this set is so that I can do a time trial of the (potentially) adapted yeast compared to nonadapted yeast grown in D2O and with DDW yeast. And if that experiment reveals that my yeast is adapted to D2O, I would like to compare yeast grown in D2O to yeast adapted to D2O and to yeast grown in and adapted to H2O.

Exciting times ahead!