D2O Adaptation Day 6: Time Trials

Yesterday’s cultures incubated very well. In just 24 hours the absorbance tripled. I don’t have enough data yet to confirm if that is high, average, or low, but I feel it is pretty decent (which is me taking an optimistic middle ground). Unrecorded yesterday, I started a DDW sample from a single colony for today’s time trial. Let’s check out the stats:

  • DDW – 3.018
  • 50% D2O (this is labeled as 50/50 in the spreadsheet) – 2.776 (started at 0.61)
  • 99% D2O – 1.873 (started at 0.618)

To setup the time trials, I inoculated 1ml of each of the above samples in 9ml of YPD (of each respective type, with 50% D2O being 4.5ml of DDW YPD and 4.5ml of D2O YPD). I will record the growth hourly via the nanodrop.

About Anthony

Advanced ape that understands logic, creativity, emotion, and expression. Displays great cognitive skills with somewhat limited communication skills. I'm some kind of new smart ape, artist extraordinaire, lover and fighter, and damned fine scientist.