Yeast Adaption Time Trials: Setup

Yesterday’s starter cultures finally started to grow so I can move ahead as planned. For now there isn’t much difference between these experiments and the time trials I did in May (see D2O1 and DDW1 categories). The only difference is that I’ll be comparing the growth amongst data sets over time and I’m also doing a long term growth sample. I’ll explain in my next post.

Here is my setup:

  1. Put 9ml of DI, DDW, and D2O YPD in 3 test tubes respectively (each in it’s own).
  2. Add 1ml of each yeast sample from the starter cultures.
  3. Starting at t=0 remove 400ul and put that in a semi-micro cuvette for nanodrop analysis. Do this every 60min.
  4. Incubate the cultures at 30C and 150rpm

Simple!

About Anthony

Advanced ape that understands logic, creativity, emotion, and expression. Displays great cognitive skills with somewhat limited communication skills. I'm some kind of new smart ape, artist extraordinaire, lover and fighter, and damned fine scientist.