Yeast Adaption Time Trials: Setup

Yesterday’s starter cultures finally started to grow so I can move ahead as planned. For now there isn’t much difference between these experiments and the time trials I did in May (see D2O1 and DDW1 categories). The only difference is that I’ll be comparing the growth amongst data sets over time and I’m also doing a long term growth sample. I’ll explain in my next post.

Here is my setup:

  1. Put 9ml of DI, DDW, and D2O YPD in 3 test tubes respectively (each in it’s own).
  2. Add 1ml of each yeast sample from the starter cultures.
  3. Starting at t=0 remove 400ul and put that in a semi-micro cuvette for nanodrop analysis. Do this every 60min.
  4. Incubate the cultures at 30C and 150rpm