I’m making slight modifications to the last reaction before I move on:

• I’m changing the ThermoCycler program temps just a little bit based on some observations I made with the machine yesterday. I noticed that running the machine on manual isn’t the same as running a program, during a program the block actually will go over (or under depending on the situation) temp while the machine measures the temp of a tube with oil in it. My measurements from yesterday did not experience this in manual mode.
• I’m also trying OneTaq in OpenPCR with some slightly modified temps, hoping that helps the reaction a little bit.

Here are my reactions: