Thermo PCR Sprint (“ThermoCycler”) Programmed T vs Recorded T

This experiment is a follow up to yesterday’s experimental results. Instead of trying to program the ThermoCycler by guessing, I decided to record the temperatures of various different T settings. Basically I would pick a T and try to get the recorded value near the T’s needed for the pALS PCR protocol.

I put the machine in manual mode so I can change the T when I needed to and I would take data points at 30 seconds for melting and annealing temperatures and at 30s and 5 minutes for extending T. This is how long I would be doing that step during the PCR reaction so it seemed to make sense. Below is a chart of the Set T and the recorded T at each interval.

Below that is the data of the recorded T.

The recorded temperatures of the PCR Sprint thermal cycler.

Figshare Data:

PCR Sprint Programmed T vs Recorded T. Anthony Salvagno. figshare.
Retrieved 18:51, Aug 23, 2012 (GMT)
http://dx.doi.org/10.6084/m9.figshare.94414

About Anthony

Advanced ape that understands logic, creativity, emotion, and expression. Displays great cognitive skills with somewhat limited communication skills. I'm some kind of new smart ape, artist extraordinaire, lover and fighter, and damned fine scientist.