SO I’ve done the temp experiments and decided to do a quick temp reading of the Thermo Sprint PCR machine this morning. That data will be up shortly. The temperatures for the PCR reactions below are based on those readings, while the T for the OpenPCR reaction (also below) are based on the readings from the experiments the past few days.
Anyways now I have two machines that are better prepared to perform this PCR reaction. Both machines are set for actual temps of ~92C, 50C, 72C and hopefully I get something that I can work with today. Reaction setup is here: