pALS PCR adjustments

So I just noticed that my reaction program was set with an extension temperature of 65C and it should have been 69C according to my notes. Adjustment made. I will then be setting up the reaction as shown below. It should be noted I’m doing an OpenPCR run with OneTaq (from NEB) and a regular PCR reaction (with Taq in the regular thermal cycler). Here are my protocols: