Gel setup:
- 50ml of 1xTAE buffer with 5ul of Sybr Safe (10000x in DMSO) and 0.4g of high quality agarose
- microwaved for 2 min
- poured into electrophoresis apparatus with 10 well comb (from Owl)
- running at 150V for ~40 min
Results:
So the PCR reaction didn’t work again. Hmmm. My first measure of adjustment is usually to adjust the annealing temperature so I’ll start there. Troubleshooting PCR is so annoying, but I want to get some product before next week so work at it I will.