Before I set up my reaction, I need to dilute my oligos from IDT from 100uM to 10uM, and dilute my pALS plasmid from 160ng/ul to 1.5ng/ul or basically a 1:100 dilution.

You may have noticed that this is the same thing I did last week. The difference is I’m using new oligos, DNA, Taq, etc. Hopefully the reaction runs better. Here is my reaction: