I am placing an order for the oligos and primers needed for Shotgun DNA Mapping. Here are the sequences I’m going to order:

• F834-dig: 5′ – TTTTCCCAGTCACGACGTTG – 3′
• R2008-bio: 5′ – CACGTAAGGTTTCAGAGATATATGGG – 3′
• F50-bio: 5′ – TGTGTCGCCCTTAGGTACGAACT – 3′
• R4000-dig: 5′ – TTCGCTCCAAGCTGGGCTGTGTG – 3′
• Top Adapter: 5′ – Phos – GCTGTCTGAATTCTAATGTAGTATAGTAATCCGCTCATCG – 3′
• Bottom 1a:
  1. 5′ – GAGCGGATXACTATACTACATTAGAATTCAGAC – 3′, where X=dT-Biotin
  2. 5′ – biotin – GAGCGGATTACTATACTACATTAGAATTCAGAC – 3′

I’m ordering from Integrated DNA Technologies. I was going to order three different versions of the bottom adapter, but decided to just get the two listed above. The one I omitted from the order is the 5′-bio with the floppy overhang (which won’t ligate to anything). Once I get my second supply of money from IMSD I’ll order that oligo.