ONS Dual Blogging: Open Notebook Platforms – Supplemental Information and Comments

Up on Science Exchange, I’ve written a post that describes the most ideal open notebook platforms and how they can be used for scientific documentation. Their design is certainly not intended for those purposes, but they are effective tools nonetheless. Here is a sample of the post:

There are consequently an array of open notebook platforms to choose from, and some better suited for certain applications than others. Five such platforms are discussed below, all capable of supporting scientific disciplines in varying aspects.

 

WordPress

 

Originally developed as a blogging platform, WordPress has become much more than that. It is the go to Content Management System (CMS) in web design, and is used for online shopping, blogs, artistic portfolios, personal websites, and even open notebooks. Personally speaking, WordPress is the most versatile platform for open notebooks and should be the model that open notebook designers look toward.

I also go on to discuss Media Wiki, Google Docs, Evernote, and Github. And you can check out the whole post here:

Open Notebook Series: Open Notebook Platforms

I think each service has a lot to offer to open notebook scientists, and with services like Evernote and Google Docs, you could certainly get away with using multiple services. Think combining Google Docs with WordPress/Media Wiki.

The most intriguing service to me is Github. If the wiki was more powerful, Github would be the ultimate notebook in my opinion. The reason is because you can upload any file type to your repository, and there is a social network, and you have access to a wiki to supplement your notebook. I haven’t played too much with the social network component, but if it is even 10% as effective as facebook it could prove very useful.

Github also makes sharing repositories easy and collaboration very easy. Anyone can fork your repository (make a copy to their repository) and if you are collaborating, pushing updates is at the discretion of the original repository’s owner. And it is all versioned in case some files get deleted.

And I’m not sure how true this is, but I’ve heard that Github is open sourced in some capacity so you may even be able to self host your own guthub repository. This would be amazing for ONS and scientists could setup their own lab site to enable collaboration amongst only the members of the lab.

And to further supplement my ONS Platform post, I offer some alternative platforms/tools to aid your open notebooking skills:

Creative Solutions to ONS

There are too many tools on the internet to keep track of, but if you don’t like the options that I mention in my post, here are a few creative alternatives that you may appreciate.

  • Flickr/Picasa – Do you want to keep writing with paper and pen? Try taking pictures of your handwritten notes and upload them to one of the many photo sharing websites. I’ve done this from time to time and upload the image files here to supplement my notebook, when I don’t want to rescribe all the work I’ve already done.
  • Social Media – The real-time capabilities of social media gives you the outlet to post what you want when you want it. Steve and I have used FriendFeed to take notes in real-time before facebook had the feature, and essentially you can do this from any social media platform available. If you use a platform like WordPress/Media Wiki, you can even embed your FriendFeed posts into your notebook and people could see your notes in real time in your native platform.
  • Tablets/SmartPhones – There are plenty of apps that let you take notes, share images/videos, bridge platforms and publish to the web right from your phone/tablet. Who says you need to be tethered to your lab pc?
  • Blogs – I’ve already talked about WordPress, which is more than a blog. But blogging services like Blogger and Tumblr offer comparable features. Blogger is trying to be more like a CMS, but still doesn’t have all the functionality of WordPress, but the Google Suite of Apps helps ease the burden quite a bit. Tumblr however is more limited in its capacity, but if you are going with just text and images for your notebook then it will suit you just fine.
  • Wikispaces – Quick and easy wiki setup in the cloud. No need to self install like Media Wiki, but also not as customizable.

And if you use a tool that I haven’t mentioned I’d love to hear about it and how you make it work for you. ONS doesn’t work if it is tedious and you don’t want to update. The goal of technology is to enhance your workflow and make life a little easier. And I’ve found that the tools and platforms I mention above and in my post at Science Exchange aid my quest for scientific domination.

IGERT Goodness

Sorry for the lack of notebooking the past few days. I’m going to assume it will continue to be infrequent through July. I feel like a faculty member and I haven’t even graduated yet! Now I know what Steve went through!

Anyways most of my time is spent in meetings and working on the IGERT proposal which is due in just a few short weeks. This week is a first draft and trying to finalize letters of support. Im doing a lot of campaigning.

I’m also working on getting the #Scifund money deposited. I’m not having issues with UNM,  the delay is all on my end but I’ll get it taken care of quickly.

Don’t worry the research will pick up shortly. If only I had a graduate student to help me out. Now how can I get one of those? …

Can you make money from open notebook science?

At the #scienceatrisk meeting in DC last week, a colleague asked me how I would make money from open notebook science. I was very surprised to say the least and I wasn’t entirely sure of the intentions of the comment. Was I being set up? Was I feeding this person the information they needed to create a business from a passion of mine? Regardless those answers, I played the game but not in the way I was expected to play.

You know me… I like to bend the rules.

So how would I (or anyone for that matter) make money from open notebook science?

Read carefully…

YOU CAN’T!

Nor should you. But before I get into all that, let me first say that if someone proves me wrong I’d be happy to help them in their endeavor (and claim NONE of the monetary rewards!). Now let’s get on with it…

I say you shouldn’t make money from open notebook science. ONS is a concept, and not really a tangible thing. You can make money from ideas by creation/production. But with ONS there is no production. The only thing I could think of making money from is education. That is, teach people about ONS, how to do it, and what’s available right now for interested parties to use. But it wouldn’t be very open if I kept that information to myself. That mentality is what put science in the predicament that it is in today. So I teach others for free!

But aside from the ethics of profiteering from ONS, it seems pretty near impossible to make money from it anyway.

  1. You can’t charge people to use something they are already afraid to use. Correct me if I’m wrong, but any business that is built on making money from a product that has no market doesn’t survive very long. With open notebooks, scientists are already afraid of being open. They are already afraid of using online tools for research purposes. And a ton of research specific tools (including notebooks) have already failed. You just can’t say “Hey, I’ve got this awesome online notebook for you to use… it’s just $100 a month for your lab!” The culture is already opposed to the incentives offered by open science. Why stack the deck against it more? We need reasons for scientists to pursue open research, not make sure they never embrace it.
  2. An online/electronic notebook is not the same as a paper notebook.Physical notebooks and pens/pencils are cheap. I may have been in the cloud for too long, but a notebook and pen combination can’t be more than $3. There aren’t too many online services that request less than $5 a month. So there is no competition with pricing. Also the entire world today is raised with writing on paper with pen/pencil. That is not the same experience as typing on a computer. Currently, open notebooks rely on the model that scientists will write some information in their paper notebook and then rewrite all that in their online notebook. This has to change. Technology is improving so scientists can go straight from experiment to the cloud. In my case, it took a few months of reprogramming (my brain that is) and this culture shift needs to happen as well. ONS systems can’t use the same workflow as paper/pen. If it does there is no competition.
  3. Data comes in all shapes and sizes. Pictures, movies, text, code, numbers, spreadsheets, files, folders, measurements, etc. You may think you can develop a complete notebook platform, but you may just make a system so complete that you make it useful to only one person. Yourself. Maybe you get lucky and you make a notebook useful for a specific group of people, biologists. The point is, in today’s world data isn’t as cut and dry as it once was. And you would need to have a very flexible interface to make sure that more than one target group could use your system. As a graphic designer, a lot of my data is .ai files and .png files. As a biologist, a lot of my data is excel spreadsheets, .jpeg, and .avi. And as a physicist my data is written as code, equations, and connections. There isn’t a single tool on the internet that can easily handle all of that. And there may never be.
  4. The open notebook needs to be flexible. This is an extension of the previous point, but if data comes in many varieties, then surely information does too. What about thought processes? Workflows? Outputs? There are too many variables and there isn’t one system that does it all well. We can’t even handle social media! Facebook, Twitter, Google+ all do the same job and all reach different audiences. The same will be said for ONS. Biologists, physicists, computer engineers, mathematicians, etc all execute research via differing methods. Should these scientists (and other fields of research not labeled in STEM) pursue open notebook science, they will require different types of notebook platforms. There is simply no way to plan and design a notebook system to meet the needs of everyone.

With all that said, why force scientists to use one platform, or force them to choose between a few options? Why not let scientists determine their own needs and be creative with how they share their science? If ONS is going to be the future of scientific research documentation, then it has to be as free flowing as possible.

Right now there are plenty of free and easy to use tools that are perfect for notebooks. And now is not the time to stifle a new movement by profiting off of it. But perhaps one day in the future (a looong time from now) there will be room to create an open notebook platform that people would WANT to pay for. And when that day comes, I’ll be excited for two reasons: (1) the culture of science has evolved for the better, and (2) I was there to get the ball rolling.

 

Dual Blogging: Why you should be an open notebook scientist

Up on Science Exchange is my latest post in the ONS series. Today’s post talks about the incentives for doing open notebook science. Here is a snippet:

The inherent transparency to ONS forces you to think more critically about your research.

 

Knowing beforehand that your research will be open for scrutiny, you’ll think twice about taking that minor shortcut in the lab that may save you twenty minutes. And if you take the shortcut and decide not to publish, you’ll be critiqued for the omission. By consequence, publishing openly in real time forces you to be careful, thorough, and explicit. No one will be able to question the integrity of your research, because the entire record will be available to anyone.

You can read the rest of the article here: Open Notebook Series: Why You Should Be An Open Notebook Scientist.

And once you are done with that, you can check out my commentary on another issue in ONS. I was asked in DC last week how I would make money from open notebooks. I replied, “I wouldn’t” which was met with some hostility. Check out the rest of my answer here: Can you make money from open notebook science?

A discourse on open notebook science: dual blogging!

I’m going to be doing an interesting experiment. I’m writing a series of guest blog entries about open notebook science for Science Exchange. The first one is already published: What is Open Notebook Science? Here is a snippet:

Ideally, every scientist could maintain an open notebook in real-time which would encompass all aspects of their research. But many fears about dealing with complete open access, conflicts with patent applications and publications, and online data overload and hamper this movement. To combat this, practitioners (like myself) encourage any form of open notebook science, even if that means uploading some information for a project from many years ago that never saw the light of day.

From what I’ve heard it’s been pretty well received and people are clamoring for the second entry which I’ve just completed a draft of and should be revised and posted either tonight or tomorrow (of course I’ll link to that).

Now here is the interesting experiment part of it. I’ll be outlining major components of ONS over at Science Exchange, and I’ll be using this notebook to go into interesting details that I wouldn’t normally be able to on that platform. I’ve already discussed one such topic here a while ago: Is there room for advertising in open notebooks?

And if you have any questions about ONS, getting started, ways to enhance your notebook, or anything of that nature feel free to leave a question/comment either via Twitter, Facebook, the comments, or via email. I’m always all over communication!

Yeast Time Trials in DI, DDW, and D2O: Trial 5 Results

Via figshare:

Yeast Hourly Growth in DI, DDW, and 99% D2O. Anthony Salvagno. figshare.
Retrieved 21:15, Jul 03, 2012 (GMT)
http://dx.doi.org/10.6084/m9.figshare.92771

Notes:

  • I had a meeting this morning regarding the IGERT I speak so frequently of. That means the timed data wasn’t so consistent in the beginning. I took my first time point about 25 minutes after I set up the experiment. And then I took my second time point 2 hours later. The raw data will show that I took the first time point about 20 minutes later, but I assure you the time difference was more than that.
  • I did take the rest of the time points an hour apart.
  • I spilled a considerable amount of the D2O sample (which was in the beaker), maybe like 30-40% of it after I took the first time point.
  • In data news, I find it really strange that the yeast in D2O didn’t grow nearly as much as it did in the past. I’m wondering if the new setup had anything to do with that.
  • Also it looks like yeast in DDW grew more than the yeast in DI. Again I’ll have to do another run to verify, but I feel like this could be a real result.
  • The added volume definitely improved the results, as the yeast remained suspended in the flasks. In the previous trials I would get considerable settling because the test tubes wouldn’t swirl as much. Much better setup.

So the moral of this story is, I’ll have to do another trial of this setup. Fine by me! Whatever it takes to get good results that are repeatable. That’s the nature of open notebook science!

An icon for open science

Some of you may have seen this picture I created in 2010.

I’m quite fond of it. I originally created it for a Science Commons t-shirt contest, which I did not win (but was told it was close, but probably not with this image). Regardless I’ve been using it ever since as my go to open science icon. I even put it in my #Scifund challenge proposal on Rockethub.

Well I realized that I haven’t been very sharing or caring with regards to this picture. That’s not intentional of course, as I don’t think there is anything that I wouldn’t be willing to share with someone unless I am eating. So here is the .png. Unfortunately I can’t upload the original .ai file, but I can upload that to figshare or something. So if you are interested in playing around with the original, I’ll upload it.

Feel free to use this image as you see fit regardless but just add a “Thanks Ant,” “Thanks IheartAnthony,” “IheartAnthony’s image. Do you?” or “Thanks @thescienceofant” to your remix or your distribution. I’ll be just fine with that! Oh and do send me a link to your remixed work. I’d love to see where you are using this or what you’ve done with it!

Yeast Time Trials in DI, DDW, and D2O: Trial 5 Setup

I set up the starter cultures yesterday. Today is the day to track the growth over time. The only differences between this experiment and the ones I’ve done in the past is that this time I’m: (1) using higher volumes of YPD (total of 25ml) and (2) only doing three samples (DI, DDW, and 99% D2O) instead of the normal five (DI, DDW, 30%, 60%, and 99% D2O). I just didn’t have enough made YPD to do the other two samples.

Here is my setup:

  1. I’m using 2 small flasks and 1 small beaker. I only have 2 flasks so the beaker is an improvisation. I think they are all 50-80ml beakers/flasks but I’ll have to verify this. (Update: They are all 50ml glassware.)
  2. I added 22.5ml of each YPD type to the glassware.
  3. I added 2.5ml of each YPD starter culture to the glassware + YPD
  4. Put in the incubator/shaker at 125rpm and 30C.
  5. Record absorbance every hour.***
    1. blank with each type of ypd (ie measure the DI YPD culture after blanking with DI YPD, etc).
    2. I’m using semi-micro cuvettes and measuring with a NanoDrop 2000c (see experiment products page)

***I had some meetings this morning so my first time point is a half hour after I started incubation. And my second time point is 2 hours later. Every point after that is 60 minutes apart. See data for explanation.

#SciFund money update

I received a form from the UNM foundation before I went to DC and I need to get it signed. I’ll be running around campus tomorrow to get it done and then once that is done I’ll be waiting for notification of how to use the UNM Banner system to start spending.

First thing to do: get thank you cards designed and printed and mailed! Then I’ll likely buy some water once I run out of supplies here. But that won’t be for a couple of weeks I think (once the money clears).

Shotgun DNA Mapping: Creating the Unzipping Construct

I have a lot of work to do with regard to organizing my thoughts for this project. It has been 1 year since I last thought about this, but it is time to restart it. I’ll explain the history of this project and where I’m going with it in future posts (this week), but for now I’m going to introduce what I need to do this week with some links for me to check out.

Here is an intro to Shotgun DNA Mapping. Go there for a crash course and some links that go further in depth than what I’m ready to divulge at this moment.

Step 1 of Shotgun DNA Mapping (SDM from here on out) is to create the unzipping construct. For that I’ll need some DNA.

There are 3 pieces of DNA required to have a completely unzip-ready object. They are:

  • The anchor – this is a 1kb/4kb (depending on the situation, kb = kilobases) double stranded sequence that is created from the PCR reaction of a plasmid. In our old cases it was pRL574, but I experimented with another sequence that I named pALS. I may start with the pRL574 plasmid to get started. This piece contains a molecule that allows us to attach the DNA to a glass surface (the microscope slide).
  • The adapter duplex – this is technically two single strands of DNA that are annealed together to create a weird double stranded piece. It is ~25bp long and there are tons of variations that I’ve experimented with. This piece contains a molecule that allows us to attach the DNA to a microsphere. It also hosts a space that allow us to essentially break the DNA so we can unzip it.
  • The unzipping DNA – this is the DNA that gets unzipped in the experiment. It can be anything essentially, but for the purposes of SDM we use yeast genomic fragments, and in the very near term I’ll be using pBR322 (a commerically available plasmid) to test the reactions and to calibrate the tweezers.

As I start to figure out what needs to be done I’ll have separate posts explaining everything about each piece. In the mean time here are a bunch of links that will help organize my thoughts:

The order of things that need to get done:

  1. I need to check my inventory. I’m not going to use this stuff for the most immediate experiments, but it will be good to know what I have. I will need to use my supplies of pALS and pRL574 and pBR322, but the adapter sequences will need to be brand new.
  2. Check the DNA sequences.
  3. Order new sequences.
  4. Get into molecular biology – PCR, annealing, ligation, gels, digestions, etc. This is where it gets exciting.

I’ll start by posting pictures and descriptions of the things that I have.