Finally the starter cultures worked! But not really in the way that I expected. The stuff that was two days old grew and none of the new cultures that I started yesterday grew. Well at least I have something to work with today. Here is my experimental setup:

1. Setup of five samples:
   1. 9ml of DI YPD + 1ml of DI starter culture
   2. 9ml of DDW YPD + 1ml of DDW starter culture
   3. 7ml of DDW YPD + 2ml of D2O YPD + 1ml of D2O starter culture (30% D2O sample)
   4. 4ml of DDW YPD + 5ml of D2O YPD + 1ml of D2O starter culture (60% D2O sample)
   5. 9ml of D2O YPD + 1ml of D2O starter culture (99% D2O sample)
2. Measuring:
   1. 400ul of each starter culture in semi-micro cuvettes – to measuring starting absorbance
   2. 400ul of each timed culture (the samples to be measured over time) in semi-micro cuvettes – to measure the time=0 value (should be close to 1/10 of the starting measurements)
   3. repeat B every hour
3. Blanking:
   1. Before each hourly measurement you need to blank the nanodrop
   2. Blank the DI samples with DI YPD (no yeast added)
   3. Blank the DDW samples with DDW YPD
   4. Blank the 99% D2O, 30% D2O, and 60% D2O samples with D2O YPD