Starter Cultures Failed Again!

So of the 6 starter cultures I made (2 of each water type: DI, DDW, D2O) only one sample grew and interestingly and amazingly enough it was the D2O sample! Well today I’m giving it one more go.With the same setup, just using a fresh batch of yeast. The yeast I bought from ATCC.org came in some solution, and I created about 20 aliquots from that solution. So from one aliquot I inoculated 4 new samples (2 DI, 1 DDW, and 1 D2O). And I streaked some from this aliquot one an agar plate.

On top of that I inoculated some of the yeast that had grown in the 1 YPD D2O culture into all the failed cultures. I just want to see if they’ll grow from an established colony.

Let’s see what happens tomorrow.

 

About Anthony

Advanced ape that understands logic, creativity, emotion, and expression. Displays great cognitive skills with somewhat limited communication skills. I'm some kind of new smart ape, artist extraordinaire, lover and fighter, and damned fine scientist.