Starter Cultures: D2O, DDW, and DI YPD

I just started three new starter cultures for another timed experiment tomorrow. Hopefully this time it goes better than last time.

Setup:

  • 10ml of YPD broth in test tubes
  • The water component of each test tube is different, one is DDW, one is DI water, and the other is 99% D20.
  • The YPD was made last week and stored in the fridge.