So before I left on travel (a week and a half ago) I received a vial of dry yeast. I’ve kept it in the fridge for 2 weeks to preserve it and today I’m going to bring it to life and start a starter culture so that this week I can do time point growth, like I did with the E. coli. Here is some information about the yeast:

• 9763 S. cerevisiae NRRL Y-567 Lot # 57640348 from ATCC

And here is some information on how to go from the powder to a liquid suspension and then to a culture (provided by ATCC):

1. Open ampoule (the glass vial with the yeast powder) according to enclosed instructions (there are no instructions).
2. From a single test tube of sterile distilled water (5 to 6ml) withdraw approx. 0.5 to 1.0ml with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile water.
4. Let the test tube sit at room temp (25C) undisturbed for at least 2 hours; longer rehydration may increase viability of some fungi.
5. Mix the suspension well. Use several drops to inoculate recommended solid or liquid medium. Include a control that receives no inoculation.
6. Incubate the inoculum at the propagation conditions recommended.
7. Inspect for growth of the inoculum strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation.

And the recommended incubation temp is 30C.