E. Coli Growth in DI, DDW, and D2O Data

From FigShare:

E. coli growth in D2O, DDW, and DI. Anthony Salvagno. Figshare.
Retrieved 01:18, Apr 26, 2012 (GMT)
hdl.handle.net/10779/e186403d944367bcbf5bdd9ed6977a88

The handle is a broken link for some reason so here is the direct link until that gets resolved.

And some notes:

  •  Right when I was collecting data for the last set, I noticed that the incubator/shaker wasn’t running. Basically it wasn’t shaking. The reason for this is that the lid technically was open. Usually I have to lean on the shaker to get it to close properly, but for some reason that wasn’t working. I then had to spend about 15 min trying to fix it (which I did), and I’ll post my fix for it tomorrow. Bicep kiss on that (Note: don’t google bicep kiss with safe search off).
  • For some reason the data at hour four regresses. By that I mean the e. coli absorption was less than it was at hour 3 for the D2O sample (and for the other two I believe). It continued to regress for hour 5, but the other two samples did not.
  • Also I haven’t analyzed the growth, but based on the numbers it appears that the cells divided faster in 33% D2O than the rest of the samples. I have no idea what’s going on here.

For tomorrow, I don’t think I’ll be redoing this experiment. So I’ll just do another starter culture and try it for Friday. I’ll also do cultures in DDW, 30% D2O, 60% D2O, and 99% D2O.

Now big important question. Should I autoclave the DDW and D2O LB Broth mixes? Keep in mind the major fear of deuterium exchange.

  • http://stevekochscience.blogspot.com Steve Koch

    I don’t think you should autoclave, since it’s steam and you need to keep the lids open, so I’d expect a lot more exchange.  I think sterile filtering (0.2 micron) (you can buy 500 ml thingy’s that work with a vacuum, or maybe even gravity) would be much better.