FT-IR Training and Experiments

Since mid December, I’ve been urging Alex to set up FT-IR training with Stephen Myers and being the go-getter that she is, she has set up a few training sessions. You can see her notes from that here.

I spoke with her at the beginning of the week to try and plan the future of these experiments. We have a couple of experiments that we would like to try out which wouldn’t be all that time consuming, and I’m leaving her in charge of all of it (she’s big time now!).

Yesterday was just step 1. We went to examine 4 samples. We wanted to analyze (3mL of each): DDW, D2O, DI, and DDW + 1uL of D2O (which was roughly equivalent to the standard mean ocean water).

I haven’t looked at the data in any real detail yet, but by eye the DDW and DI looked very similar, and there was almost no difference between that and DDW+1ul D2O (but I thought it was slightly discernable). Because of this we tried 3mL of DDW with 70ul of D2O added (which is roughly 2% by volume D2O), and this was obviously different in profile (but barely). As per usual, the pure D2O sample was very different.

Hopefully Alex will get the data on FigShare today (I could do it myself easily, but I would like her to experience the awesomeness).

Mini rant in 3… 2… 1… I gotta get her to be on top of her notebook a little better. I keep telling her to notebook frequently about everything, and that she just needs to get into the habit of doing it. She agrees but then doesn’t notebook. Grrrr… I was hoping to do this post yesterday but alas Alex didn’t post until after my bedtime so here it is now. Once she enters the lab I’ll be all over her like a tiger on a gazelle licking lilacs in the wilted meadow of frozen tundra. With regards to posting to figshare, of course. Rant over.

Anyways the plan for the project is as follows:

  1. Understand the spectroscopy results as they correlate to the physical properties of the water molecules (H2O and D2O and HOD). This will be major literature searching on Alex’s part with aid by myself.
  2. FT-IR analysis of D2O/H2O mixtures. Koch and I think there could be some really interesting results/studies in the mixture range around the amount that is naturally occurring. So I want to do a broad study of different D2O amounts in DDW from 0 up to 100% so we can understand how the peaks shift from H2O bonds to DOH bonds to D2O bonds.
  3. FT-IR analysis of DDW time dependence. D-exchange is a real thing. It happens all the time and rapidly, but how long does it take for DDW to reach the levels of deuteration of naturally occuring water? This is something I’d like to know. And something we hope to find out.

So with all that said, I’ll be linking to Alex’s notebook with regards to these experiments in the future and add some minor notes myself here. In the meantime I will just twiddle my thumbs until those data sets get uploaded to figshare…

  • Anonymous

    One correction, we examined 5 samples (3mL of each), plus an additional scan (initially) of the empty cuvette for background:

    DDW, DDW + 1uL D2O (roughly equivalent to SMOW), DDW + 70uL D2O (because the 1uL looked so close to DDW), DI, D2O.

    Anthony is right, for some strange reason, I have a hard time blogging every day. I’m aware of this and have asked Anthony to help push me in the right direction. I think this is really important and nothing but good things can come of it. It’s kind of like keeping a diary, which I have never been very consistent with. Time to break old bad habits and create new ones.