Enhanced Image of Arabidopsis in DDW (day 3)

I decided to try and look at the full resolution image (found here) and think I noticed something. Generally I don’t look at the full resolution pictures, but I decided to try it here because I noted that you could see a radicle (preroot) but the image isn’t clear. So I blew it up and you can slightly make it out in the center of the image shown above.

The thing that I noticed isn’t that though. What I noticed is that if you look at the seeds to the right, it looks as if there are some hairs already growing out of the seed. Now I’m not declaring this to be the case because it is really tough to tell, but my brain is giving me the impression that there are root hairs, and I thought I’d open it up for discussion. What do you think? If it isn’t clear from the above image (which will get bigger if you click it) then click the original image link (linked above) and let me know what you think.

  • http://stevekochscience.blogspot.com Steve Koch

    What does it look like with your eyes?  Especially now that some time has elapsed.  I can’t see well, even from the high-res photo.  I think given that Arabidopsis is so much smaller it’s probably time to buy a macro lens, probably about $500.  Maybe an SLR too?  Maybe we have Addgene money left to do so?  It’s unrestricted, but anyway, this is the perfect sharing purpose.  I’d say for sure get a macro lens right away so you can share better what you see with your eyes (and actually with macro lens, probably even better).  What do you think?  In the meantime (aka Sunday / Monday or even early Tuesday), I think it would be really cool to know whether you see a lot more root hairs from Arabidopsis in DDW than DI. We’d still have the huge issue of characterizing the water, but it would be very nice to have the phenotypical result from both Arabidopsis and tobacco.

    • http://www.iheartanthony.com Anthony Salvagno

      It’s too small to see with my eyes (and my eyes aren’t very good anyways). When I go in today I’ll give it a good look. As for the Addgene money, I don’t think there is much left. Maybe a hundred dollars? I’d have to combine accounts.

      Do you think it would be worth getting a profesional grade dslr and macro lens? Maybe about $5000 combined? I’ll look into the pricing, but that is what I’d guess it would be.

      • http://stevekochscience.blogspot.com Steve Koch

        Definitely not worth $5000.  I think the macro lens even with the D40 will be wonderful.  The D40 DSLR body is probably only around $300 nowadays, and it is excellent.  It doesn’t have a lot of pixels, but with the correct lens, you don’t need a lot of pixels.  So, I’d say the combination of macro lens (maybe $700 for excellent lens) + body will only be about $1000.  You can also look into getting a shutter trigger to reduce vibration.

        • http://www.iheartanthony.com Anthony Salvagno

          So remote shutter, macro and new d40 ( so u can have urs back)?

          • http://stevekochscience.blogspot.com Steve Koch

            Yes.  macro lens highest priority. http://www.amazon.com/Nikon-60mm-2-8G-Micro-Nikkor-Cameras/dp/B0013A1XDY/ref=sr_1_3?ie=UTF8&qid=1320603856&sr=8-3 that’s a good place to start.  I am worried, though, about whether arabidopsis will still be too small.  maybe read the reviews or look for photos on wikimedia commons that have been taken with that lens?  many photos on the commons have the camera information included (at least i think so)